The concentration of a sodium hydroxide solution is determined by titrating it against a sample of potassium h?
Q. The concentration of a sodium hydroxide solution is determined by titrating it against a sample of potassium hydrogen phthalate (KHP). KHP is a weak acid with one hydrogen and a molar mass of 204.22 g/mole. In the titration, 20.46 mL of the sodium hydroxide was used to react with 0.1082 grams of KHP. What is the molarity of the NaOH?
Asked by John - Wed May 5 19:04:08 2010 - - 1 Answers - 0 Comments
A. 0.1082 g KHP * (1 mol / 204.22 g) = 5.298 * 10^-4 mol KHP 5.298 * 10^-4 mol KHP * (1 mol NaOH / 1 mol KHP) = 5.298 * 10^-4 mol NaOH 5.298 * 10^-4 mol NaOH / 20.46 ml * (10^3 mL / 1 L) = 2.590 * 10^-2 M NaOH, or 0.02590 M
Answered by David R - Wed May 5 19:14:20 2010
Q. The concentration of a sodium hydroxide solution is determined by titrating it against a sample of potassium hydrogen phthalate (KHP). KHP is a weak acid with one hydrogen and a molar mass of 204.22 g/mole. In the titration, 20.46 mL of the sodium hydroxide was used to react with 0.1082 grams of KHP. What is the molarity of the NaOH?
Asked by John - Wed May 5 19:04:08 2010 - - 1 Answers - 0 Comments
A. 0.1082 g KHP * (1 mol / 204.22 g) = 5.298 * 10^-4 mol KHP 5.298 * 10^-4 mol KHP * (1 mol NaOH / 1 mol KHP) = 5.298 * 10^-4 mol NaOH 5.298 * 10^-4 mol NaOH / 20.46 ml * (10^3 mL / 1 L) = 2.590 * 10^-2 M NaOH, or 0.02590 M
Answered by David R - Wed May 5 19:14:20 2010
How do you find the experimentally determined H3O ion concentration,M?
Q. How do you find the experimentally determined H3O ion concentration,M?
Asked by jere - Tue Nov 20 10:32:04 2007 - - 1 Answers - 0 Comments
A. measure the pH H3O+ = 10^-pH
Answered by ferrous lad - Tue Nov 20 10:46:30 2007
Q. How do you find the experimentally determined H3O ion concentration,M?
Asked by jere - Tue Nov 20 10:32:04 2007 - - 1 Answers - 0 Comments
A. measure the pH H3O+ = 10^-pH
Answered by ferrous lad - Tue Nov 20 10:46:30 2007
How to determine concentration of of chlorophyll using Beer Lambert Law?
Q. Apart from the use of a formula related to a given extraction solvent, is there any other way in which concentration of chlorophyll can be determined, making use of the Beer Lambert Law? Any help would be greatly appreciated. Thankyou!
Asked by jane 62 - Thu Nov 9 16:05:02 2006 - - 1 Answers - 0 Comments
A. The Beer-Lambert Law forms the basis of Spectroscopy, an analytical tool for measuring concentrations. Spectrophotometers are instruments used in laboratories to measure concentrations. The most common is a UV/Vis spectrophotometer. This instrument passes light(in this case UV or visible light) through a fixed length of solution and measures the radiation absorbed at a particular wavelentgh. Thus the instrument gives an absobance reading of the solution. Since the thickness or length of the solution is constant (the solution is placed in a glass cuvette, a little glass square that is of known length/thickness) from the Beer-Lambert law, the absorption is proportional to the concentration. So, you prepare say three solutions of… [cont.]
Answered by Smokeybones - Thu Nov 9 21:14:01 2006
Q. Apart from the use of a formula related to a given extraction solvent, is there any other way in which concentration of chlorophyll can be determined, making use of the Beer Lambert Law? Any help would be greatly appreciated. Thankyou!
Asked by jane 62 - Thu Nov 9 16:05:02 2006 - - 1 Answers - 0 Comments
A. The Beer-Lambert Law forms the basis of Spectroscopy, an analytical tool for measuring concentrations. Spectrophotometers are instruments used in laboratories to measure concentrations. The most common is a UV/Vis spectrophotometer. This instrument passes light(in this case UV or visible light) through a fixed length of solution and measures the radiation absorbed at a particular wavelentgh. Thus the instrument gives an absobance reading of the solution. Since the thickness or length of the solution is constant (the solution is placed in a glass cuvette, a little glass square that is of known length/thickness) from the Beer-Lambert law, the absorption is proportional to the concentration. So, you prepare say three solutions of… [cont.]
Answered by Smokeybones - Thu Nov 9 21:14:01 2006
The pH of a chemical solution is determined by using the formula below where [H+] is the concentration of hydr?
Q. The pH of a chemical solution is determined by using the formula below where [H+] is the concentration of hydrogen in moles per liter. pH = -log10[H+] What is the pH of a solution for which [H+] is 0.001?
Asked by BellKatt - Thu Apr 8 20:50:10 2010 - - 3 Answers - 0 Comments
A. log ten is in subscript because it is the base. the base is always the denominator in logs. you just divide the log of the outside number by the log of the base : -log(.001) / log(10) = 3
Answered by mark - Thu Apr 8 20:53:35 2010
Q. The pH of a chemical solution is determined by using the formula below where [H+] is the concentration of hydrogen in moles per liter. pH = -log10[H+] What is the pH of a solution for which [H+] is 0.001?
Asked by BellKatt - Thu Apr 8 20:50:10 2010 - - 3 Answers - 0 Comments
A. log ten is in subscript because it is the base. the base is always the denominator in logs. you just divide the log of the outside number by the log of the base : -log(.001) / log(10) = 3
Answered by mark - Thu Apr 8 20:53:35 2010
How is the concentration of a buffer solution determined?
Q. How is the concentration of a buffer solution determined?
Asked by charm - Sat Dec 1 01:50:21 2007 - - 3 Answers - 0 Comments
A. You need to refine your question. A buffer is actually a mixture of two species: a weak acid and its conjugate base, so there are actually two concentrations that contribute to the pH of a buffer. A buffer is a solution that has a relatively constant pH over a wide range of added acid or base. You may want to check out the Henderson-Hasselbalch equation. It is used to compute the pH of a buffer based on the concentrations of weak acid and conjugate base and the Ka for the weak acid.
Answered by pisgahchemist - Sat Dec 1 02:18:02 2007
Q. How is the concentration of a buffer solution determined?
Asked by charm - Sat Dec 1 01:50:21 2007 - - 3 Answers - 0 Comments
A. You need to refine your question. A buffer is actually a mixture of two species: a weak acid and its conjugate base, so there are actually two concentrations that contribute to the pH of a buffer. A buffer is a solution that has a relatively constant pH over a wide range of added acid or base. You may want to check out the Henderson-Hasselbalch equation. It is used to compute the pH of a buffer based on the concentrations of weak acid and conjugate base and the Ka for the weak acid.
Answered by pisgahchemist - Sat Dec 1 02:18:02 2007
The concentration of hydrogen peroxide in a solution is determined by titrating a 12.0 sample of the solution?
Q. with permanganate ion. 2MnO _(4^- ) + 5H_2 O_2 + 6H^+ 2 Mn ^(2+) + 5O_2 + 8H_2 O If it takes 16.4 of 0.125 solution to reach the equivalence point, what is the molarity of the hydrogen peroxide solution?
Asked by faiz - Fri Aug 13 10:26:34 2010 - - 1 Answers - 0 Comments
Q. with permanganate ion. 2MnO _(4^- ) + 5H_2 O_2 + 6H^+ 2 Mn ^(2+) + 5O_2 + 8H_2 O If it takes 16.4 of 0.125 solution to reach the equivalence point, what is the molarity of the hydrogen peroxide solution?
Asked by faiz - Fri Aug 13 10:26:34 2010 - - 1 Answers - 0 Comments
Determine concentration of chloride ions in tap water by titration?
Q. The concentration of chloride ions in tap water is 21.3 parts per million (ppm). Show, with a calculation, why it is not advisable to determine the chloride ion concentration of tap water by titrating it with silver nitrate solution. Deduce the percent error (large/small/no change) incurred and give the reason why. Help please everybody!! Thank you so much ^^
Asked by Gvriz - Sun Sep 21 05:30:40 2008 - - 1 Answers - 0 Comments
A. Because the water wikll become undrinkable
Answered by LoanShark - Sun Sep 21 06:40:12 2008
Q. The concentration of chloride ions in tap water is 21.3 parts per million (ppm). Show, with a calculation, why it is not advisable to determine the chloride ion concentration of tap water by titrating it with silver nitrate solution. Deduce the percent error (large/small/no change) incurred and give the reason why. Help please everybody!! Thank you so much ^^
Asked by Gvriz - Sun Sep 21 05:30:40 2008 - - 1 Answers - 0 Comments
A. Because the water wikll become undrinkable
Answered by LoanShark - Sun Sep 21 06:40:12 2008
By whic technique r ink extractions typicaly analysed? How may the concentration of th components b determined
Q. By whic technique r ink extractions typicaly analysed? How may the concentration of th components b determined
Asked by viperdesignss - Thu Dec 6 05:27:12 2007 - - 1 Answers - 0 Comments
A. Chromatography
Answered by Shagger - Thu Dec 6 14:06:56 2007
Q. By whic technique r ink extractions typicaly analysed? How may the concentration of th components b determined
Asked by viperdesignss - Thu Dec 6 05:27:12 2007 - - 1 Answers - 0 Comments
A. Chromatography
Answered by Shagger - Thu Dec 6 14:06:56 2007
The concentration of a hydrogen peroxide solution can be conveniently determined?
Q. by titration against a standardized potassium permanganate solution in an acidic medium according to the following equation: 2 MnO4- + 5 H2O2 + 6 H+ 5 O2 + 2 Mn2+ + 8 H2O If 36.44 mL of a 0.01652 M KMnO4 solution are required to completely oxidize 25.00 mL of a H2O2 solution, calculate the molarity of the H2O2 solution.
Asked by cheergurliej11 - Tue Feb 12 00:06:38 2008 - - 1 Answers - 0 Comments
A. moles MnO4- = 0.01652 x 0.03644 = 0.0006020 mol have 5/2 = 2.5 x 0.0006020 = 0.001505 mol H2O2 Vol = 36.44ml + 25.00ml = 61.44 ml Molarity = 0.001505 / 0.06144 = 0.02450 mol /L, H2O2
Answered by Michael E - Tue Feb 12 00:15:29 2008
Q. by titration against a standardized potassium permanganate solution in an acidic medium according to the following equation: 2 MnO4- + 5 H2O2 + 6 H+ 5 O2 + 2 Mn2+ + 8 H2O If 36.44 mL of a 0.01652 M KMnO4 solution are required to completely oxidize 25.00 mL of a H2O2 solution, calculate the molarity of the H2O2 solution.
Asked by cheergurliej11 - Tue Feb 12 00:06:38 2008 - - 1 Answers - 0 Comments
A. moles MnO4- = 0.01652 x 0.03644 = 0.0006020 mol have 5/2 = 2.5 x 0.0006020 = 0.001505 mol H2O2 Vol = 36.44ml + 25.00ml = 61.44 ml Molarity = 0.001505 / 0.06144 = 0.02450 mol /L, H2O2
Answered by Michael E - Tue Feb 12 00:15:29 2008
How do you determine the concentration of a solution given the amount precipitated?
Q. I added 6mL of sulfate solution to 4mL of an unknown concentration of barium ion solution and precipitated 0.01g BaSO4. How do you determine the unknown concentration? Ksp for BaSO4 is 5.E-10. I added 6mL of 0.1M sulfate solution to 4mL of an unknown concentration of barium ion solution and precipitated 0.01g BaSO4. How do you determine the unknown concentration? Ksp for BaSO4 is 5.E-10.
Asked by Chris Compton - Fri Jul 23 22:21:42 2010 - - 1 Answers - 0 Comments
A. 0.01 g BaSO4 / 233.4 g/mole = 4.3x10^-5 mole 4.3x10^-5 mole / 0.004 L = .011 M Ba^+2 (You gave the Ksp for BaSO4; an insignificant amout of BaSO4 dissolves.)
Answered by skipper - Fri Jul 23 22:58:21 2010
Q. I added 6mL of sulfate solution to 4mL of an unknown concentration of barium ion solution and precipitated 0.01g BaSO4. How do you determine the unknown concentration? Ksp for BaSO4 is 5.E-10. I added 6mL of 0.1M sulfate solution to 4mL of an unknown concentration of barium ion solution and precipitated 0.01g BaSO4. How do you determine the unknown concentration? Ksp for BaSO4 is 5.E-10.
Asked by Chris Compton - Fri Jul 23 22:21:42 2010 - - 1 Answers - 0 Comments
A. 0.01 g BaSO4 / 233.4 g/mole = 4.3x10^-5 mole 4.3x10^-5 mole / 0.004 L = .011 M Ba^+2 (You gave the Ksp for BaSO4; an insignificant amout of BaSO4 dissolves.)
Answered by skipper - Fri Jul 23 22:58:21 2010
How do you determine the concentration?
Q. If you have 10.0 mL of solution A, 80.0 mL of distilled water, and 10.0 mL of solution B, how do you determine the concentration of solution A? Why is it necessary to use distilled water and not tap water in reactions?
Asked by aSnxbByx113 - Tue Jan 23 20:35:30 2007 - - 2 Answers - 0 Comments
A. What you are doing with those two solutions and the distilled water? Are they ALL three being mixed together? If all three are being mixed together, then the concentration of solution A in the whole mixture is... A / (A+B+Distilled Water) 10 / (10+80+10) .. which is... 10/100 ... which is the same as saying... 10% So the the solution would be 10% concentration of A. Learn the outline of how this is done. Don't just take the answer. Math is important and many people don't even realize this until it's late in the game. Study hard now so you don't have to work as hard later! The reason you use only distilled water, is because tap water is treated with chlorine and has minerals - like the residue left in the bathtub (that's lime a… [cont.]
Answered by James K - Tue Jan 23 20:53:12 2007
Q. If you have 10.0 mL of solution A, 80.0 mL of distilled water, and 10.0 mL of solution B, how do you determine the concentration of solution A? Why is it necessary to use distilled water and not tap water in reactions?
Asked by aSnxbByx113 - Tue Jan 23 20:35:30 2007 - - 2 Answers - 0 Comments
A. What you are doing with those two solutions and the distilled water? Are they ALL three being mixed together? If all three are being mixed together, then the concentration of solution A in the whole mixture is... A / (A+B+Distilled Water) 10 / (10+80+10) .. which is... 10/100 ... which is the same as saying... 10% So the the solution would be 10% concentration of A. Learn the outline of how this is done. Don't just take the answer. Math is important and many people don't even realize this until it's late in the game. Study hard now so you don't have to work as hard later! The reason you use only distilled water, is because tap water is treated with chlorine and has minerals - like the residue left in the bathtub (that's lime a… [cont.]
Answered by James K - Tue Jan 23 20:53:12 2007
How can the Beer Lambert Law be used to determine the concentration of a substance in solution?
Q. If you know the molar absorbance coefficient of a particular substance, how can the Beer-Lambert Law be used to determine the concentration of a substance in solution?
Asked by heartybarbeque - Thu Apr 24 04:23:08 2008 - - 1 Answers - 0 Comments
A. Beer Lambert Law: A=E.b.c You have the the solution...measure its absorbance(A), you know the absorbace coefficient(E), you know the path length of the cell used to measure absorbance(b). Now rearrange the equation to get c... c = A/(E.b) done kthnx
Answered by minip2087 - Thu Apr 24 04:30:25 2008
Q. If you know the molar absorbance coefficient of a particular substance, how can the Beer-Lambert Law be used to determine the concentration of a substance in solution?
Asked by heartybarbeque - Thu Apr 24 04:23:08 2008 - - 1 Answers - 0 Comments
A. Beer Lambert Law: A=E.b.c You have the the solution...measure its absorbance(A), you know the absorbace coefficient(E), you know the path length of the cell used to measure absorbance(b). Now rearrange the equation to get c... c = A/(E.b) done kthnx
Answered by minip2087 - Thu Apr 24 04:30:25 2008
why cant you determine the concentration of NaCl based on absorption of light as apposed to CoCl2?
Q. If you can make a graph with concentration on the x axis and absorbance on the y to determine concentration of say, CoCl2, why cant you use that method (finding concentration based on absorption of light) with NaCl? is it because it's two nonmetals? - if so, please explain thanks! =)
Asked by hre - Wed Dec 9 11:09:50 2009 - - 1 Answers - 0 Comments
A. CoCl2 has a color... therfor we know it absorbs visible light... so we can make a graph with concentration on the x axis and absorbance on the y to determine concentration NaCl has not a color so we know that it does not absorb visible light at room twemp so we can't make a graph with concentration on the x axis and absorbance on the y to determine concentration however if we introduce the solution into a flame, it will emit/ & absorb light, so then we can make a graph with concentration on the x axis and absorbance on the y to determine concentration emitter: absorber:
Answered by Steve O - Wed Dec 9 13:39:31 2009
Q. If you can make a graph with concentration on the x axis and absorbance on the y to determine concentration of say, CoCl2, why cant you use that method (finding concentration based on absorption of light) with NaCl? is it because it's two nonmetals? - if so, please explain thanks! =)
Asked by hre - Wed Dec 9 11:09:50 2009 - - 1 Answers - 0 Comments
A. CoCl2 has a color... therfor we know it absorbs visible light... so we can make a graph with concentration on the x axis and absorbance on the y to determine concentration NaCl has not a color so we know that it does not absorb visible light at room twemp so we can't make a graph with concentration on the x axis and absorbance on the y to determine concentration however if we introduce the solution into a flame, it will emit/ & absorb light, so then we can make a graph with concentration on the x axis and absorbance on the y to determine concentration emitter: absorber:
Answered by Steve O - Wed Dec 9 13:39:31 2009
How can you determine the concentration of a bleach solution (besides looking on the label)?
Q. I am an anesthetist in Liberia and we process some equipment with bleach for disinfection. The CDC recommends 5.25% solution for this, but the solution we have here (supposedly) is 18%. I can do the math to make a 5.25% soln, but we have no actual docs that say it's 18% and there is some question whether the manufacturer is watering it down. How can I determine approx concentration? I have access to basic lab reagents (of a medical lab), some biological staining dyes (meth blue, indigo carmine, safranin, etc) and fairly accurate measuring equipment, but it's been a long time since I've taken quantitative chem. Any suggestions?
Asked by tommeltj - Mon Apr 16 15:54:13 2007 - - 3 Answers - 0 Comments
A. Add excess potassium iodide to a measured volume sample of the bleach. Then titrate the iodine released against standardised sodium thiosulphate solution, using starch solution as an indicator. Unlike other titrations, starch is added towards the end of the titration process. This is a standard technique for measuring the concentration of bleach in the UK. ClO- + 2I- + 2H+ ---> I2 + Cl- + H2O then I2 + 2(S2O3)2- ---> (S4O6)2- + 2I-
Answered by Gervald F - Mon Apr 16 16:15:29 2007
Q. I am an anesthetist in Liberia and we process some equipment with bleach for disinfection. The CDC recommends 5.25% solution for this, but the solution we have here (supposedly) is 18%. I can do the math to make a 5.25% soln, but we have no actual docs that say it's 18% and there is some question whether the manufacturer is watering it down. How can I determine approx concentration? I have access to basic lab reagents (of a medical lab), some biological staining dyes (meth blue, indigo carmine, safranin, etc) and fairly accurate measuring equipment, but it's been a long time since I've taken quantitative chem. Any suggestions?
Asked by tommeltj - Mon Apr 16 15:54:13 2007 - - 3 Answers - 0 Comments
A. Add excess potassium iodide to a measured volume sample of the bleach. Then titrate the iodine released against standardised sodium thiosulphate solution, using starch solution as an indicator. Unlike other titrations, starch is added towards the end of the titration process. This is a standard technique for measuring the concentration of bleach in the UK. ClO- + 2I- + 2H+ ---> I2 + Cl- + H2O then I2 + 2(S2O3)2- ---> (S4O6)2- + 2I-
Answered by Gervald F - Mon Apr 16 16:15:29 2007
How to determine the concentration of plasmid DNA?
Q. You want to determine the concentration of plasmid DNA (i.e. it is double-stranded) in a stock tube. You dilute it by mixing 22 microlitres of your stock DNA with water to obtain a final volume of 1 mL. You obtain an absorbance reading at 260 nm of 0.15 using a standard 1-cm-wide cuvette. What is the concentration of DNA in your original stock tube?
Asked by fefee_33 - Tue Sep 30 15:25:24 2008 - - 2 Answers - 0 Comments
A. An OD260 of 1 is equivalent to 50 micrograms/ml. Concentration of DNA was 0.15 X 50 micrograms/ml = 7.5 micrograms/ml. You diluted 22 microlitres into 1000 Your initial concentration was (1000/22) X 7.5 micrograms/ml = 340.91 micrograms/ml.
Answered by Vincent M - Tue Sep 30 15:45:20 2008
Q. You want to determine the concentration of plasmid DNA (i.e. it is double-stranded) in a stock tube. You dilute it by mixing 22 microlitres of your stock DNA with water to obtain a final volume of 1 mL. You obtain an absorbance reading at 260 nm of 0.15 using a standard 1-cm-wide cuvette. What is the concentration of DNA in your original stock tube?
Asked by fefee_33 - Tue Sep 30 15:25:24 2008 - - 2 Answers - 0 Comments
A. An OD260 of 1 is equivalent to 50 micrograms/ml. Concentration of DNA was 0.15 X 50 micrograms/ml = 7.5 micrograms/ml. You diluted 22 microlitres into 1000 Your initial concentration was (1000/22) X 7.5 micrograms/ml = 340.91 micrograms/ml.
Answered by Vincent M - Tue Sep 30 15:45:20 2008
how does one determine the concentration of spilled acid in a well? do you use titration or simply indicators?
Q. does one use titration or simply use indicators to determine concentration of acid in water?
Asked by Joshua - Sun Jun 13 18:19:43 2010 - - 1 Answers - 0 Comments
Q. does one use titration or simply use indicators to determine concentration of acid in water?
Asked by Joshua - Sun Jun 13 18:19:43 2010 - - 1 Answers - 0 Comments
A student wishes to determine the concentration of a solution of HCl.?
Q. A student wishes to determine the concentration of a solution of HCl. The student adds 10.00 mL of the HCl solution to 150 mL of water, adds an indicator, and titrates with .0965 M NaOH. If the titration requires 32.45 mL of base, what is the concentartion of the HCl solution?
Asked by EE - Sun Aug 26 16:21:15 2007 - - 1 Answers - 0 Comments
A. 10 mL x M = 32.45 mL x 0.0965 HCl = 0.3131 M
Answered by skipper - Sun Aug 26 16:55:37 2007
Q. A student wishes to determine the concentration of a solution of HCl. The student adds 10.00 mL of the HCl solution to 150 mL of water, adds an indicator, and titrates with .0965 M NaOH. If the titration requires 32.45 mL of base, what is the concentartion of the HCl solution?
Asked by EE - Sun Aug 26 16:21:15 2007 - - 1 Answers - 0 Comments
A. 10 mL x M = 32.45 mL x 0.0965 HCl = 0.3131 M
Answered by skipper - Sun Aug 26 16:55:37 2007
How to determine initial concentration from molarity and ml?
Q. I am using 4.0 M acetone. If I prepare a solution that is diluted to a total volume of 50.0 mL, using 10 mL of acetone, what is the initial concentration of acetone (in units of M)? There are other factors involved, but I think this is everything needed to answer this question.
Asked by Ella - Wed Oct 7 16:58:54 2009 - - 1 Answers - 1 Comments
A. I suspect you are reading the question incorrectly. If you add solvent to the acetone the solution will become diluted. This would result in a FINAL concentration. The original concentration of the acetone will remain at 4.0 M. However the new, diluted concentration would be (10 ml/ 50 ml) x 4.0 M = 0.80 M is the diluted concentration.
Answered by stush - Wed Oct 7 17:07:24 2009
Q. I am using 4.0 M acetone. If I prepare a solution that is diluted to a total volume of 50.0 mL, using 10 mL of acetone, what is the initial concentration of acetone (in units of M)? There are other factors involved, but I think this is everything needed to answer this question.
Asked by Ella - Wed Oct 7 16:58:54 2009 - - 1 Answers - 1 Comments
A. I suspect you are reading the question incorrectly. If you add solvent to the acetone the solution will become diluted. This would result in a FINAL concentration. The original concentration of the acetone will remain at 4.0 M. However the new, diluted concentration would be (10 ml/ 50 ml) x 4.0 M = 0.80 M is the diluted concentration.
Answered by stush - Wed Oct 7 17:07:24 2009
How do you determine the concentration of ammonia in a window washing solution?
Q. Basically, I need the procedure of how to calculate the concentration of ammonia in Windex.
Asked by Andrea K - Fri Apr 25 00:22:58 2008 - - 4 Answers - 0 Comments
A. can you explain the reason you'd like to know this?
Answered by Chixdiggit - Fri Apr 25 00:32:49 2008
Q. Basically, I need the procedure of how to calculate the concentration of ammonia in Windex.
Asked by Andrea K - Fri Apr 25 00:22:58 2008 - - 4 Answers - 0 Comments
A. can you explain the reason you'd like to know this?
Answered by Chixdiggit - Fri Apr 25 00:32:49 2008
How to determine original cell concentration by colony count?
Q. I have 50 colonies with a dilution factor of 10^-6. 50 uL of this dilution was plated originally and left over night resulting in the 50 colonies. How do I determine the original cell concentration in units of cells/mL? I was thinking of using C1V1=V1V2, but cannot figure out how what to do with colony number.
Asked by FuFu - Wed Oct 21 16:20:15 2009 - - 1 Answers - 0 Comments
A. I think you assume that each colony is based on a single cell. As you used 50ul and got 50 colonies, it's easy, you would have 1 cell per ul in your dilution. Now as you diluted it, you would have 10^6 cells in one ul. Which does seem too much, but that's what your numbers indicate.
Answered by tigri - Wed Oct 21 16:29:27 2009
Q. I have 50 colonies with a dilution factor of 10^-6. 50 uL of this dilution was plated originally and left over night resulting in the 50 colonies. How do I determine the original cell concentration in units of cells/mL? I was thinking of using C1V1=V1V2, but cannot figure out how what to do with colony number.
Asked by FuFu - Wed Oct 21 16:20:15 2009 - - 1 Answers - 0 Comments
A. I think you assume that each colony is based on a single cell. As you used 50ul and got 50 colonies, it's easy, you would have 1 cell per ul in your dilution. Now as you diluted it, you would have 10^6 cells in one ul. Which does seem too much, but that's what your numbers indicate.
Answered by tigri - Wed Oct 21 16:29:27 2009
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